Vitrification

1. Oocyte Cryopreservation

Oocyte cryopreservation is the process of freezing and storing eggs for future use. It is commonly used for women undergoing medical treatments that may affect their fertility or those who wish to delay childbearing.

Procedure

1. Ovarian Stimulation:
   • Hormonal Treatment: Women receive hormonal medications (usually FSH and LH) to stimulate the ovaries to produce multiple eggs.
   • Monitoring: Ultrasound and blood tests monitor follicle development and hormone levels.
2. Egg Retrieval:
   • Procedure: Once eggs are mature, they are retrieved through a minor surgical procedure called transvaginal ultrasound-guided aspiration.
   • Anesthesia: Usually performed under sedation or general anesthesia.
3. Cryopreservation:
   • Preparation: The retrieved eggs are processed and evaluated for maturity.
   • Cryoprotectant Addition: Eggs are treated with cryoprotectants to protect them from ice crystal formation during freezing.
   • Freezing: Eggs are frozen and stored in a cryogenic tank until they are needed in the future.